Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

High resolution with short run times and good recovery. High capacity at high flow rates. Separations can be scaled up to production levels. Prepacked for convenience and reproducibility. 1/16” fittings for convenient connections to AKTA design systems.

General description

HiLoad^® 26/600 Superdex^® 75 prep grade are prepacked 53 mL XK columns designed for high-resolution preparative gel filtration chromatography separating small proteins and polynucleotides.Superdex^® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr ﹤ 10 000 (Superdex^® 30 pg), Mr 3 000 to 70 000 (Superdex^® 75 pg), and Mr 10 000 600 000 (Superdex^® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Legal Information

Superdex is a registered trademark of Cytiva

HiLoad is a registered trademark of Cytiva

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Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Suitability

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides