HiScreen Ni Fast Flow, Cytiva, 28-9782-44

Code: GE28-9782-44 D2-231

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

HiScreen columns a...


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Your Price
€208.10 EACH
€255.96 inc. VAT

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

HiScreen columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The chromatography media used in HiScreen columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.HiScreen Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose® 6 Fast FLow which has high binding capacities forhistidine-tagged Proteins and minimal Ni2+ leakage.

Features and Benefits

High binding capacity, approx. 40 mg/mL medium and negligible leakage of Ni2+. Prepacked, ready-to-use columns for convenient process development. Excellent choice for method optimization and parameter screening due to the 10 cm bed height. Easy connection in series to achieve 20 cm bed height. Small bed volume gives fast results and minimal sample/buffer consumption Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

General description

HiScreen columns prepacked with Ni Sepharose® 6 Fast FLow for method optimization and parameter screening for purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC).

Legal Information

Sepharose is a registered trademark of Cytiva

HiScreen is a trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

average diameter90 µm
bed size7.7 mm × 100 mm
bed volume4.7 mL
capacity~40 mg binding capacity(histidine-tagged protein)
cleaning2-14(Ni2+-stripped medium.)
column I.D.7.7 mm
manufacturer/tradenameCytiva 28-9782-44
matrix6% cross-linked agarose
packagingpkg of 1 ea
parameter22 psi, 1.5 bar (22 psi) (Over the Packed Bed During Operation)
particle size45-165 µm
shelf lifePlease be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
suitabilitysuitable for bioprocess medium
working range3-12(Ni2+-stripped medium.)
This product has met the following criteria to qualify for the following awards:



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