Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

HiScreen^™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen^™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The chromatography media used in HiScreen^™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.HiScreen^™ Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose^® 6 Fast FLow which has high binding capacities forhistidine-tagged Proteins and minimal Ni2+ leakage.

Features and Benefits

High binding capacity, approx. 40 mg/mL medium and negligible leakage of Ni2+. Prepacked, ready-to-use columns for convenient process development. Excellent choice for method optimization and parameter screening due to the 10 cm bed height. Easy connection in series to achieve 20 cm bed height. Small bed volume gives fast results and minimal sample/buffer consumption Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

General description

HiScreen^™ columns prepacked with Ni Sepharose^® 6 Fast FLow for method optimization and parameter screening for purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC).

Legal Information

Sepharose is a registered trademark of Cytiva

HiScreen is a trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)