Analysis Note

ControlJurkat lysate

Application

Research Sub CategoryImmunological Signaling

This Anti-IRAK1 Antibody, C-terminus is validated for use in WB for the detection of IRAK1.

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IRAK1, also known as Interleukin-1 receptor associated kinase 1, belongs to the tyrosine kinase related (TKL) family and is involved with mRNA stabilization and activation of NF-kappa B. IRAK1 is located in all tissue as 2 isoforms, with 1 binding quickly and then degrading while isoform 2 is activated by kinase, resulting in a slower more sustained response to cytokines. Phosphorylation of IRAK1 leads to recruitment of the adapter protein PELI1. IRAK1 utilizes 2 pathways, the first one allows for mediation of IL-1 inflammatory responses when TRAF6 is bound, which activates NF-kappa B, thereby inhibiting I kappa B alpha. A second pathway does not involve TRAF6 and results in the stabilization of mRNA.

Immunogen

Epitope: C-terminus

KLH-conjugated synthetic linear peptide corresponding to the C-terminal region of IRAK1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified rabbit polyclonal in buffer containing PBS and 0.09% sodium azide.

Quality

Evaluated by western blot in jurkat lysate.

Western Blot Analysis: A 1:50-1:500 dilution of this antibody detected IRAK1 on jurkat lysate.

Specificity

This antibody recognizes the C-terminal region of IRAK1.

Storage and Stability

Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20°C.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~77 kDa