Analysis Note

ControlHeLa nuclear extract or EGF-treated whole HeLa cell extract.

Application

ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).

Dot blotting:
This antibody has been shown by an outside laboratory to be specific for the phospho-Ser714 immunogen peptide, but not the unphosphorylated peptide, by dot blotting (1:500).

Anti-Phospho-Dnmt1(Ser714) Antibody is a rabbit polyclonal antibody for detection of Phospho-Dnmt1(Ser714) also known as CXXC finger protein 9, DNA (cytosine-5-)-methyltransferase 1 & DNA MTase HsaI has been validated in WB, ELISA, DB.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes may be linked with development of cancer. Three families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of Dnmt1 causes global demethylation of genomic DNA and embryonic lethality. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. Phosphorylation of Ser714 is induced by EGF, but the role of this modification is unknown.

Immunogen

The immunogen was a KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser714 of human Dnmt1.

Epitope: Ser714

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified

Purified rabbit polyclonal in buffer containing 0.09% NaN3.

Format: Purified

Quality

Western Blot Analysis:
1:250 dilution of this antibody detected Dnmt1 on 10 µg HeLa nuclear extract

Specificity

Not tested in other species.

This antibody recognizes human Dnmt1 phosphorylated on Ser714.

Storage and Stability

Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~184 kDa