Analysis Note

ControlCOS-7+EGF cell lysates untreated and lambda phosphatase treated.

Application

Anti-phospho-WAVE2 (Ser343) Antibody detects level of phospho-WAVE2 (Ser343) & has been published & validated for use in WB.

Western Blot Analysis: A previous lot of this antibody was used to detect endogenous phospho-WAVE2 (Ser343) in COS-7 and HMECs following EGF stimulation ± pretreatment with U0126 (Mendoza, M.C., et al. (2011). Mol Cell. 41(6):661-671).

Research CategoryCell Structure

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Wiskott-Aldrich Syndrome Protein (WASP)-family Protein Member 2 (known as WAVE2) is found in many cells and tissues, with strong expression in peripheral blood leukocytes. WAVE2 is involved in signaling RTKs and small GTPases. As a whole, the WAVE family of proteins have a general function for regulating the actin cytoskeleton in various tissues.

Immunogen

Epitope: Phosphorylated Ser343

KLH-conjugated linear peptide corresponding to human WAVE2 phosphorylated at Ser343.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in COS-7+EGF cell lysates untreated and lambda phosphatase-treated.

Western Blot Analysis: A 1:500 dilution of this antibody detected phospho-WAVE2 (Ser343) in 10 µg of COS-7+EGF cell lysates untreated and lambda phosphatase treated.

Specificity

This antibody recognizes WAVE2 phosphorylated at Ser343.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~75 kDa observed.
An uncharacterized band appears at ~103 kDa in some lysates.