Analysis Note

ControlRat hippocampus and cerebellum tissue

Application

Research CategorySignaling

Anti-cAMP Antibody detects level of cAMP & has been published & validated for use in IH(P).

Research Sub CategoryGPCR, cAMP/cGMP & Calcium Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cyclic adenosine monophosphate or cAMP is an intracellular by-product of ATP by adenylene cyclase when G-protein Coupled Receptors (GPCR) bind various ligands on the cell surface. Cyclic AMP acts as a second messenger for signal transduction across the cell membrane and is a part of kinase activation. It regulates the effects of glucagon and adrenaline along with the movement of calcium ions through ion channels. Since it is affected directly by GPCR activation, cAMP is studied as a means to monitor GPCR in the discovery of therapeutic drugs.

Immunogen

2′O-succinyl cyclic AMP conjugated to BSA

Linkage

Replaces: MABN1026

Physical form

Unpurified rabbit serum

Diluted serum presented as liquid in PBS containing no preservatives.

Quality

Evaluated by Immunohistochemistry in rat hippocampus and cerebellum tissue.
Immunohistochemistry Analysis: 1:100 dilution of this lot detected cAMP in rat hippocampus and cerebellum tissue.

Specificity

Specific for cAMP with no cross reaction to other nucleotides at concentrations to 1 mM. Reactive with cAMP fixed in tissues or acetylated cAMP. Not reactive with free, non-acetylated, cAMP in RIA or EIA assays.

Storage and Stability

Maintain at -20°C in undiluted aliquots from date of shipment. Avoid repeated freeze/thaw cycles.