Analysis Note

ControlIncluded Positive Antigen Control: Catalog # 12-301, non-stimulated A431 lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.

Application

Anti-Lamin A/C Antibody, clone 14 detects level of Lamin A/C & has been published & validated for use in IC & WB.

Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect Lamin A/C in human endothelial cells.

Research CategoryCell Structure

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nuclear lamins are composed of the type V intermediate filament proteins. Often referred to as nucleoskeletal proteins they play a key role in nuclear integrity, positioning of nuclear pores, and overall nuclear size and shape. They also play several key functional roles in the maintenance and propagation of the genome--replication, transcription-- as well as the disassembly and reassembly of the nucleus during cell division. In vitro studies have shown that dimers are the basic building blocks of higher order lamin structures and in low concentrations lamins are distributed throughout the nucleoplasm. In humans, there are two types of lamins: A-type lamins (lamins A and C), found primarily in differentiated cells, and B-type lamins (lamins B1 and B2), found in all nucleated cells. Nuclear lamins are involved in a number of essential nuclear functions, including nuclear envelope assembly and disassembly during cell division, DNA synthesis, transcription, and apoptosis. Nuclear lamins have been found to co-localize with DNA synthesis sites.

Immunogen

Peptide from human lamin A/C corresponding to amino acids 398 to 490.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MABE481

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing 50% storage buffer (20 mM sodium phosphate, pH 7.5, 0.15 M NaCl, 1 mg/mL BSA, 0.09% sodium azide) and 50% glycerol. Store at -20°C.

Format: Purified

Quality

Evaluated by western blot on RIPA lysates from A431 cells.

Western Blot Analysis:
0.5-2 µg/mL of this antibody detected Lamin A/C in 20 µg of RIPA lysates from A431 cells.

Specificity

Recognizes Lamin A, MW ~74 kDa and Lamin C, MW ~65 kDa.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

~74/65 kDa