Analysis Note

ControlNIH/3T3 cell lysate

Application

Anti-HIRA Antibody, clone WC119 is a Mouse Monoclonal Antibody for detection of HIRA also known as HIR (histone cell cycle regulation defective) homolog & has been validated in WB & IP.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin BiologyChaperones

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Hall, C., et al. (2001). Molecular and Cellular Biology. 21(5):1854-1865.)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

HIRA (TUP1 like enhancer of split protein 1) is a protein localized primarily in the nucleus. It functions as a histone chaparone to preferentially place histone H3.3 on nucleosome. It is broadly expressed in a variety of tissues and is expressed during embryogenesis. Data suggests HIRA plays a part in mechanisms of transcriptional regulation similar to that played by yeast HIR1 and HIR2 together. The HIRA protein has been shown to interact with HIRIP3, HIRIP5, histones H2B and H4, and PAX3. HIRA may play a part in the etiology of the DiGeorge syndrome (DGS), a developmental disorder. The clinical features of this disease include absence or hypoplasia of the thymus and parathyroid glands, cardiovascular malformations, facial dysplasia, a cleft palate and mental retardation.

Immunogen

Epitope: Unknown

GST-tagged recombinant protein corresponding to human HIRA.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 0.44 µg/ml of this antibody detected HIRA on 10 µg of NIH/3T3 cell lysate.

Specificity

This antibody recognizes HIRA.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 112 kDa