Application

Flow Cytometry Analysis: A representative lot from an independent laboratory detected CLEC9A in mouse Conventional Dendritic cells and on most Plasmacytoid Dendritic cells (Caminschi, I., et al. (2008). Blood. 112(8):3264-3273.).

Research Sub CategoryImmunoglobulins & Immunology

Detect CLEC9A using this rat monoclonal antibody, Anti-CLEC9A Antibody, clone 10B4 validated for use in western blotting & Flow Cytometry.

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CLEC9A, also known as Dendritic cell natural killer lectin group receptor 1 (DNGR-1), and encoded by the gene Clec9a/Dngr-1, functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. CLEC9A functions as a SYK-coupled C-type lectin receptor to mediate sensing of necrosis by the principal dendritic-cell subset involved in regulating cross-priming to cell-associated antigens. CLEC9A recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediates the cross-presentation of dead-cell associated antigens in a Syk-dependent manner. CLEC9A is localized to the plasma membrane. CLEC9A is expressed in dendritic cells and has multiple isoforms; isoform 4 is expressed at high levels by CD8+ dendritic cells and at low levels by plasmacytoid DCs but not by other hematopoietic cells.

Immunogen

KLH-conjugated linear peptide corresponding to mouse CLEC9A.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in mouse lymph node tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CLEC9A in 10 µg of mouse lymph node tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~27 kDa and ~16 kDa observed. Uniprot describes five isoforms at 27 kDa, 27 kDa, 30 kDa, 20 kDa, and 17 kDa