Analysis Note

ControlMouse spleen tissue lysate

Application

Detect ASC using this Anti-ASC Antibody, clone 2EI-7 validated for use in WB, FC.

Research Sub CategoryApoptosis - Additional

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, Bcl10, Apaf-1, ARC, caspase-2 and caspase-9. A novel CARD domain containing protein has been identified in human and mouse and designated ASC and TMS1. Irregular expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells. Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.

Immunogen

Epitope: Unknown

Full length recombinant human ASC.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot on mouse spleen tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected ASC in mouse spleen tissue lysate.

Specificity

This antibody recognizes ASC.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~22 kDa