Analysis Note

ControlHeLa cell lysate

Application

Immunoprecipitation:
Cited in Thoma, C., et al. (2008) RNA. 14:1579-1589, and Buchet-Poyau, K., et al.(2007) Nuc. Acids Res. 35:1289-1300 using a representative lot.

Immunocytochemistry:
Cited in Afonina, E, et al. (1998) J. Biol. Chem., 273: 13015-21 (1998) using a representative lot.

Research Sub CategoryRNA Metabolism & Binding Proteins

Research CategoryEpigenetics & Nuclear Function

Anti-PABP 1, clone 10E10 is a Mouse Monoclonal Antibody for detection of PABP 1 also known as PABP 1, Poly(A)-binding protein 1, poly(A) binding protein cytoplasmic 1 & has been validated in WB, ICC & IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Poly(A)-binding protein (PABP 1) is an abundant protein that interacts with the 3′ poly(A) tail of most eukaryotic mRNAs. In vivo this protein is involved in many aspects of mRNA metabolism, including mRNA stability, translationally coupled mRNA turnover, protein biosynthesis, and ribosome biogenesis. PABP1 shuttles between the cytoplasm and the nucleus and may bind to cytoplasmic RNA sequences other than poly(A). PABP1 accumulates in the nucleus if transcription is inhibited, suggesting that nuclear export is coupled to active transcription.

Immunogen

Epitope: Unknown

Recombinant protein corresponding to human PABP 1.

Linkage

Replaces: 05-847

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected PABP 1 in 10 µg of HeLa cell lysate.

Specificity

This antibody recognizes PABP 1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 71 kDa