Influence of tips on the performance of the pipette and the results
Within the scientific community, a rising number of experiments published cannot be reproduced by other groups. Incorrect pipette handling (e.g. holding the pipette at a wrong angle during liquid aspiration) may be one reason for this. A second source of error often not taken seriously is plastics.
Consumables may lead to problems with analysis results, e.g. due to leachable, as well as incorrect pipetting volumes, due to bad shape or geometry of the tips. This may result in non-reproducible data if experiments are repeated by other groups using tips other than recommended by manufacturer.
Some problems with pipette tips are obvious like:
• Tips must be pushed powerfully onto the pipette cone to achieve efficient tip fit
• Banana-shaped tips make it difficult to fill a plate while using a multichannel pipette
• Pipetting of volumes below 1 μL on a solid surface is impossible because the liquid drop sticks to the outside of the tip
In the same way that only the tip of an iceberg can be seen above water level, several other problems with pipette tips rather stay unknown. One example is decreased pipette accuracy when using tips not recommended by the pipette supplier. All pipette users should know when using tips not delivered by the pipette supplier, the manufacturer`s declaration or certificate of conformity does not apply.
Select the right tip
Make your everyday work easier with epT.I.P.S.® (Eppendorf Totally Integrated Pipetting System). These tips are manufactured to extremely high quality and purity standards and are designed to cover all your needs.
When choosing a matching Eppendorf pipette and tip you can be confident that your calibration is within permissible error tolerance. Check our selection chart to find the tip that fits to your pipette
Selection chart
• Eppendorf Xplorer® / Xplorer® plus - See range