Analysis Note

Recovery of labeled RNA: >80% (poly [A], approximately 500 bases)Retention of unincorporated mononucleotides: >99%Exclusion limit for RNA: ﹤72 basesSample size: up to 100 µlTime required: 10 minutes

Application

Quick spin columns for radiolabeled RNA purification has been used in digoxigenin (DIG labeled RNA probe synthesis and is also used to purify synthesized RNA.

Ready-to-use disposable column units designed to quickly and efficiently separate small (>72 bases) unlabeled or radioactively labeled RNA from small molecules. Quick Spin columns are designed for use in low-speed, swinging-bucket centrifuges.Remove unincorporated precursors from DNA labeled by nick translation, end labeling, polymerization reactions, and other labeling techniques. Use the highly purified radiolabeled DNA in Southern blotting and other downstream applications.

General description

Quick Spin Columns are produced with material shown to have a ≥80% RNA recovery.All the tedious, time-consuming steps involved in preparing columns have already been performed. Quick Spin Columns are pre-packed, pre-swollen, and quality tested to ensure: maximal retention of unincorporated nucleotides (≥99%) absence of DNase and RNase contamination according to the current Quality Control procedures.

ContentsPre-packed, pre-swollen, pre-spun columns.Suspension of Sephadex G-50 in STE buffer (10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM NaCl).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Principle

Quick Spin columns contain gel filtration matrices which allow large molecules (e.g., DNA or RNA) to pass through quickly while retaining small molecules (e.g., nucleotides). The Quick Spin format improves the molecular sieving concept by using centrifugation to separate DNA or RNA rapidly and cleanly from small contaminants.