Application

Enantioprobe (R)-4 is one of 16 fully functionalized, enantiomeric fragment probes developed in the Cravatt lab. Combining fragment-based ligand discovery (FBLD) with chemical proteomics, the enantioprobe library assesses ligandability across proteomes. Each enantioprobe contains a structurally variable fragment for interaction with proteins, photoactivatable diazirine for UV-induced covalent protein labeling, and bioorthogonal alkyne handle for detection, enrichment, and identification. Of the eight enantiomeric pairs, each differs by one stereocenter, and comparing stereoselective fragment-protein interactions between the pairs simplifies validation of authentic protein-binding events. Enantioprobe (R)-4′s paired fragment is available as Enantioprobe (S)-4 (cat#913189).Together, the 16 enantioprobes support ligandability studies in living cells, a significant method for development of chemical probes and lead discovery efforts to find binders for traditionally ″undruggable″ protein targets. This strategy is also compatible with multiplexing for higher throughput.

Supporting reagents: Fluorophore-conjugated azide tags for SDS-PAGE in-gel profiling: 910147, 760757, 760765, 777315, 777323 Azide-biotin tags for enrichment: 901765, 914134, 914460, 762024, 900912, 900891, QBD10825) Azide capture solid supports: 900957, 742627 Streptavidin for biotin enrichment: S6940, E5529, E2513, 69203, 16-126, S1638 Mass spectrometry and Multiplexing

Other Notes

Technology Spotlight: Proteomic Ligandability AssessmentExpedited mapping of the ligandable proteome using fully functionalized enantiomeric probe pairsA chiral trick to map protein ligandability