Protein A High Performance MultiTrap, Cytiva, 28-9031-33

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use. Flexible format, with protocols for classic elution and cross-link method. Elution conditions formatted for both electrophoresis and LC-MS analysis workflows. Fast binding kinetics and high capacity provides high yield. Easy scale-up with HiTrap^® Protein A HP prepacked columns. Prepacked with Protein A Sepharose^® High Performance for coupling of antibodies of IgG subclasses. MultiTrap^™ 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.

General description

Protein A Sepharose^® High Performance has high affinity for the Fc region of IgG from a variety of species.Protein A Sepharose^® High Performance has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). Remember to order the collection plates for washing and elution of the enriched protein.There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

Legal Information

Sepharose is a registered trademark of Cytiva

MultiTrap is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva