Analysis Note

Specific Activity: Affinity Precipitation Assay: 10 µg of this lot when added with 10 µL glutathione agarose, precipitated Rac-GTP from 3T3 and A431 cell lysates that were loaded in vitro with GTPγS. GDP treated of 3T3 and A431 lysates served as negative controls. Precipitated Rac-GTP was detected by immunoblot analysis using 1 &micor;g/mL anti-Rac (Catalog # 05-389) and secondary goat anti-mouse HRP antibody (Catalog # 12-349) on a Western Blot.

General description

Product Source: GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1, expressed in E. coli.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

300 µg of PAK-1 PBD, GST in 150 µL of PBS, pH 7.4, 0.5 mM PMSF, containing 20% glycerol.

Quality

Routinely evaluated by G-protein activity assay

Storage and Stability

Stable for 1 year at -20C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.