Application
Chromatin Immuno-Percipitation (ChIP) analysis Nucleosome preparation Removal of nucleic acids present in protein preparation Reduction of viscosity of cell lysates during non-mechanical cell lysis preparation RNA sequencing and transcriptomic profiling
Biochem/physiol Actions
Hydrolyzes 5′-phosphodiester bonds of DNA.
General description
Nuclease Micrococcal (MNase) is the extracellular nuclease of Staphylococcus aureus. The enzyme is an endo-exonuclease .This Endo-exonuclease preferentially digests single-stranded nucleic acids. Thus, nuclease micrococcal activity results in the production of mononucleotides and oligonucleotides with terminal 3′-phosphates. Nuclease Micrococcal is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved.Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes
Other Notes
Note: One µmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a δA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.
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