Application

Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

Incubation: 10 minutes with IHC Select™ Detection Kits.

NSE has been prediluted for use as the primary antibody with Chemicon′s IHC Select™ Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier′s IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR2014-6.

This IHC Select Anti-Neuron-Specific Enolase Antibody, prediluted, clone BBS/NC/VI-H14 is validated for use in IH(P) for the detection of Neuron-Specific Enolase.

Immunogen

Gamma-gamma enolase purified from human brain

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Monoclonal mouse antibody supplied in liquid format as tissue culture supernatant, diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% Kathon as a preservative.

Format: Purified

Specificity

Reacts with human gamma-gamma enolase (Mr 46000). This antibody does not cross react with alpha or beta subunits of NSE.