Application

This Anti-Nerve Growth Factor-β Antibody is validated for use in ELISA, WB, IH, FUNC for the detection of Nerve Growth Factor-β.

Immunohistochemistry at 1:500-1:5,000 (see suggested protocol).

Immunoblotting at 1:500-1:5,000.

ELISA at 1:500-1:5,000.

Inhibition of biological activity in vitro at 1:10-1:50.
BIOLOGICAL ACTIVITY: This antibody will completely inhibit the survival and neurite outgrowth actions of mouse NGF in chicken dorsal root ganglion neurons in vitro.

Use neat for in vivo animal studies.

Optimal working dilutions must be determined by end user.

Research CategoryNeuroscience

Research Sub CategoryNeurochemistry & Neurotrophins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Mouse nerve growth factor-beta purified from salivary glands.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1119

Physical form

Whole rabbit antisera. Lyophilized. Reconstitute with 250 µL of sterile distilled water.

Specificity

Nerve growth factor-beta (NGF). Less than 1% cross-reactivity against recombinant human NT3, NT4 and BDNF by ELISA.

Storage and Stability

Maintain at -20°C to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.