Application

Monoclonal Anti-Cy3/Cy5-Biotin antibody produced in mouse was used to label miR-107 in human breast cancer cell lines by confocal microscopy.

Monoclonal Anti-Cy3/Cy5 Biotin antibody produced in mouse has also been used in: enzyme linked immunosorbent assay (ELISA)immunocytochemistrydot blot assayprotein microarraysimmunofluorescenc

Biochem/physiol Actions

Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.

Cyanine dyes are small, soluble in aqueous solution and has tolerance to dimethyl sulfoxide (DMSO). They are more photostable than fluorescein, has high molar extinction coefficients and favourable quantum yields. Monoclonal antibodies to cyanine 3 (Cy3) and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Cy3/Cy5 (mouse IgG2a isotype), Biotin conjugate is derived from the CY-96 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a mixture of proteins labelled with cyanine 3 (Cy3) or Cy5. Cyanine dyes are ionized polymethine dyes.

Immunogen

mixture of proteins labeled with Cy3/Cy5.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody recognizes Cy3 and Cy5 conjugated to proteins.