Biochem/physiol Actions

Reversible: yes

Product competes with ATP.

Primary TargetMyosin light chain kinase (Mlck)

Target K_i: 300 nM against myosin light chain kinase

Cell permeable: yes

General description

A cell-permeable, potent, reversible, ATP-competitive, and selective inhibitor of myosin light chain kinase (K_i = 300 nM). Inhibits protein kinase A (K_i = 21 µM)and protein kinase C (K_i = 42 µM) at much higher concentrations.

A cell-permeable, potent, reversible, ATP-competitive, and selective inhibitor of myosin light chain kinase (K_i = 300 nM). Inhibits protein kinase A (K_i = 21 µM) and protein kinase C (K_i = 42 µM) at much higher concentrations.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Krarup, T., et al. 1998. Am. J. Physiol. 275, C239.Saitoh, M., et al. 1987. J. Biol. Chem.262, 7796.

Packaging

1 mg in Plastic ampoule

Preparation Note

Further dilute with aqueous buffer just prior to use.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.

Warning

Toxicity: Standard Handling (A)