Application

Immunocytochemistry on HeLa and COS7 cells

Immunohistochemistry on cold methanol fixed tissue.

Optimal working dilutions must be determined by end user.

Detect the Mannosidase II protein using this Anti-Mannosidase II, For Golgi Apparatus identification validated for use in ICC & IHC.

Research CategoryEpitope Tags & General Use

Research Sub CategoryOrganelle & Cell Markers

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: For Golgi Apparatus identification

Purified peptide from human mannosidase II.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Rabbit serum. Liquid with 0.02% sodium azide.

Specificity

Mannosidase II. This antibody is a useful marker for golgi apparatus identification.

Storage and Stability

Maintain unopened vial at -70C for up to 6 months. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2-8C° for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8C°. For storage at -20C°, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70C° for up to 6 months. Avoid repeated freeze/thaw cycles.