Application
Detect Laminin using this Anti-Laminin Antibody validated for use in ELISA, IP, WB, IH.
Immunoblot: 1:500-1:2000
ELISA: 1:4000-1:8000
Immunohistochemistry: 1:50-1:200
Immunoprecipitation: Condition must be determined by the end user
Optimal working dilutions must be determined by the end user.
Immunogen
Full length human laminin. Immunization occurred after single-band purity was assessed by SDS-PAGE.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form
Affinity purified immunoglobulin. Liquid at 1 mg/mL in 0.125 M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 with 0.01% Sodium Azide.
Format: Purified
Specificity
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins collagen and non-collagen extracellular matrix proteins to remove unwanted reactivities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI collagens or fibronectin. Non-specific cross reaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible.
Storage and Stability
The undiluted antibody solution is stable for approximately 6 months stored between 2 and 8° C. For extended storage mix with an equal volume of glycerol, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
This product has met the following criteria to qualify for the following awards: