Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetMobile ion carrier for Ca2+

General description

Highly specific for divalent cations (Ca²⁺ > Mg²⁺ >> Sr²⁺ = Ba²⁺). More effective than antibiotic A23187 as a mobile ion carrier for Ca²⁺. Complexes with Ca²⁺ between pH 7 and 9.5, causing significant absorption in the UV range; however, in contrast to A23187 (Cat. No. 100105), it is not fluorescent. Useful for studies of Ca²⁺ transport across biological membranes and measurement of cytoplasmic free Ca²⁺. Induces apoptotic neuronal degeneration in embryonic cortical neurons. Also induces cell cycle arrest in the G₁ interval of mature Burkitt lymphoma cell lines.

Absorption max. (280 nm, MeOH): 18,600 M^-1cm^-1.

Highly specific for divalent cations and more effective than A23187 (Cat. No. 100105) as a mobile ion carrier for Ca²⁺. Complexes with Ca²⁺ between pH 7 and 9.5. In contrast to A23187, it is not fluorescent. Useful for studies of Ca²⁺ transport across biological membranes and measurement of cytoplasmic free Ca²⁺. Induces apoptotic neuronal degeneration in embryonic cortical neurons and cell cycle arrest in the G₁ phase in mature Burkitt lymphoma cell lines.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Aagaard-Tillery, K.M., and Jelinek, D.F. 1995. J. Immunol.155, 3297.Takei, N., and Endo, Y. 1994. Brain Res. 652, 65.Dupuy d'Angeac, A., et al. 1993. J. Cell Physiol.154, 44.Lee, H.C. 1993. J. Biol. Chem.268, 293.Kuznetsov, G., et al. 1992. J. Biol. Chem. 267, 3932.Toeplitz, B.K., et al. 1979. J. Am. Chem. Soc.101, 3344.Liu, C.M., and Hermann, T.E. 1978. J. Biol. Chem.253, 5892.

Packaging

Packaged under inert gas

1, 5, 10 mg in Plastic ampoule

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 year at 4°C.

Warning

Toxicity: Harmful (C)