Biochem/physiol Actions

Primary Targetchymotrypsin-like activity of the multicatalytic proteinase complex (MCP; 20S proteasome) in HT4 cells

Product does not compete with ATP.

General description

Cell-permeable inhibitor of the chymotrypsin-like activity of the multicatalytic proteinase complex (MCP; 20S proteasome) in HT4 cells. Causes the accumulation of ubiquitinated proteins in neuronal cells. Prevents NF-κB activation in response to TNFα and okadaic acid through inhibition of IκBα degradation, thereby interfering with induction of iNOS in macrophages.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Griscavage, J.M., et al. 1996. Proc. Natl. Acad. Sci. USA93, 3308.Mori, S., et al. 1995. J. Biol. Chem.270, 29447.Figueiredo-Pereira, M.E., et al. 1994. J. Neurochem.63, 1578.Traenckner, E.B., et al. 1994. EMBO J.13, 5433."

Packaging

Packaged under inert gas

5 mg in Plastic ampoule

Physical form

A 50 mM (5 mg/162 µl) solution of Proteasome Inhibitor I (Cat. No. 539160) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Sequence

Z-Ile-Glu(OtBu)-Ala-Leu-CHO

Warning

Toxicity: Irritant (B)