Analysis Note

Specific Activity: Chemicon′s human IL-1beta is fully biologically active when compared to standards.The ED(50) as determined by the dose-dependent stimulation of the proliferation of murine D10S cells is ﹤0.001 ng/mL

Application

For most in vitro applications, human IL-1beta is fully biological activity in the concentration range of 0.1 to 10.0 ng/mL. Responding cells are (partial list): Leukocytes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Interleukin-1beta (IL-1beta) is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T-cells. Human IL-1beta is a 17.3 kDa protein containing 153 amino acid residues.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Sterile filtered and lyophilized from 10 mM Tris, pH 8.2 with no preservatives.

Storage and Stability

The lyophilized human IL-1beta, though stable at room temperature, is best stored desiccated at -20°C. After a quick spin, reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into other buffered solutions. Reconstituted human IL-1beta should be stored in undiluted aliquots at -20°C for up to six months.