Forskolin; Coleus forskohlii in DMSO

General description

The major cell-permeable diterpene isolated from the Indian plant Coleus forskohlii. At low doses, it acts as a positive inotropic agent. At higher doses, it serves as a hypotensive and vasodilatory agent due to its actions as a smooth muscle relaxant. No major side effects are observed at effective doses. Forskolins pharmacological activities are due to its activation of adenylate cyclase (EC₅₀ = 4 µM), resulting in increased cAMP levels. The exact mechanism of forskolins positive inotropic effect is unknown but may be related to a cAMP-dependent increase in Na⁺ permeability that results in indirect augmentation of Ca²⁺ release. Inhibits MAP kinase in rat renal mesangial cells (IC₅₀ = 25 µM). Also acts as a Hh pathway antagonist. Shown to inhibit apoptosis in cerebellar granule cells and to induce apoptosis in resting human B lymphocytes.

The major cell-permeable diterpene isolated from the Indian plant Coleus forskohlii. At low doses, it acts as a positive inotropic agent. At higher doses, it serves as a hypotensive and vasodilatory agent due to its actions as a smooth muscle relaxant. No major side effects are observed at effective doses. Forskolins pharmacological activities are due to its activation of adenylate cyclase (EC₅₀ = 4 µM), resulting in increased cAMP levels. The exact mechanism of forskolins positive inotropic effect is unknown but may be related to a cAMP-dependent increase in Na⁺ permeability that results in indirect augmentation of Ca²⁺ release. Inhibits MAP kinase in rat renal mesangial cells (IC₅₀ = 25 µM). Also acts as a Hh pathway antagonist. Shown to inhibit apoptosis in cerebellar granule cells and to induce apoptosis in resting human B lymphocytes. The solid form of this compound (Cat. No. 344270) is also available.

The major cell-permeable diterpene isolated from the Indian plant Coleus forskohlii.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

D′Orazio, J.A., et al. 2006. Nature443, 340.Noveen, A., et al. 1996. Biochem. Biophys. Res. Commun.219, 180.Galli, C., et al. 1995. J. Neurosci.15, 1172.Li, X., et al. 1995. Am. J. Physiol.269, C986.Lomo, J., et al. 1995. J. Immunol.154, 1634.Uneyama, H., et al. 1993. J. Biol. Chem.268, 168.Laurenza, A., et al. 1989. Trends Pharmacol. Sci.10, 442.Adashi, E.Y., and Resnick, C.E. 1986. J. Cell. Biochem.31, 217.Seamon, K.B., and Daly, J.W. 1986. Adv. Cyclic Nucleotide Protein Phosphorylation Res.20, 1.Huang, R., et al. 1982. Cyclic Nucleotide Res.8, 385.Metzger, H., and Lindner, E. 1981. IRCS Med. Sci. Biochem. Cardiovasc. System Pharmacol.9, 99.

Packaging

5 mg in Glass bottle

Packaged under inert gas

Physical form

A 50 mM (5 mg/244 µl) solution of Forskolin, Coleus forskohlii (Cat. No. 344270) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Irritant (B)