Application

Western blot: 1-10 µg/mL using ECL. The antibodies detected ~140 kDa band in rat liver extracts.

ELISA: 1:10,000-1:100,000, using 50-100ng of control peptide/well.

Optimal working dilutions must be determined by end user.

Research CategorySignaling

Detect FLT-4 using this Anti-FLT-4 Antibody validated for use in ELISA & WB.

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

20 amino acid peptide sequence within the cytoplasmic, C-terminus of mouse Flt-4

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Liquid in PBS, containing 0.1% BSA. Affinity purified immunoglobulin.

Specificity

Recognizes mouse Flt-4 (mouse 1363-aa and human 1298-aa) a receptor protein tyrosine kinase. It is type 1 membrane protein, a receptor for VEGF-C and is expressed in adult lung, and liver, and in fetal liver, brain, intestine, and placenta. Flt-4 is also known as vascular endothelial growth factor receptor 3 (VEGFR3).

SPECIES REACTIVITIES: The peptide sequence used is relatively conserved in rat (85%) and human (73%) Flt-4/VEGFR3. No significant sequence homology is seen with VEGFR1, VEGFR2, or other tyrosine kinases. Antibody crossreactivity in other species is not established.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze and thaw.