Biochem/physiol Actions

Cell permeable: no

Reversible: no

Primary TargetDiacylglycerol Kinase

Product does not compete with ATP.

General description

Potentiates the activity of protein kinase C by inhibiting diacylglycerol metabolism. Inhibits diacylglycerol kinase (IC₅₀ = 2.8 µM) without affecting phosphodiesterase or phosphatidylinositol kinase. Potentiates this effect in studies of signal-transducing inositol lipid metabolism. Inhibits human platelet DAG kinase in intact cells (IC₅₀ = 3.6 µM) and in isolated membranes (IC₅₀ = 6.7 µM). In porcine thymus cytosol it inhibits the 80 kDa DAG kinase (IC₅₀ = 10 µM) but has no effect on the 150 kDa DAG kinase. Weak dopamine D₂ and histamine H₁ antagonist and strong serotonin-S₂ antagonist.

Potentiates the activity of protein kinase C by inhibiting diacylglycerol metabolism. Inhibits diacylglycerol kinase (IC₅₀ = 2.8 µM) without affecting phosphodiesterase or phosphatidylinositol kinase. Weak dopamine D₂ and histamine H₁ antagonist and strong serotonin S₂ antagonist.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Goin, M., et al. 1993. FEBS Lett. 316, 68.Ohtsuka, T., et al. 1990. J. Biol. Chem.265, 15418.de Chaffoy de Courcelles, D., et al. 1985. J. Biol. Chem.260, 15762.

Packaging

5 mg in Plastic ampoule

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

Warning

Toxicity: Irritant (B)