Application

Immunohistochemistry: 1:1,000-1:2,500 glutaraldehyde required for reactivity. (0.5%-2.5% is typical; fluorescence detection is not recommended. Blocking is with 3% normal serum with 0.1-0.2% triton X-100 usually. Primary antibody is diluted only in TBS or PBS with out blocking or triton X-100. Incubations are usually 3 hours 37C or 4C overnight. Detection is by ABC or PAP for best results. Not recommended for paraffin embedded tissues.

ELISA: 1:2500-1:5000

Optimal working dilutions must be determined by the end user.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

Anti-Citrulline Antibody is a Rabbit Polyclonal Antibody for the detection of Citrulline & has been validated in ELISA & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Citrulline-Glutaraldehyde-BSA

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Rabbit antiserum. Liquid with 0.05% sodium azide

Specificity

Citrulline in glutaraldehyde fixed tissues or cells.



The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:



Compound Cross-reactivity ratio

Citrulline-G-BSA 1

Arginine-G-BSA 1/>100,000

Glutamine-G-BSA 1/>100,000

Ornithine-G-BSA 1/>100,000



Abbreviations:



(G) Glutaraldehyde

(BSA) Bovine Serum Albumin

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.