Immunohistochemistry: 1:1,000-1:2,500 glutaraldehyde required for reactivity. (0.5%-2.5% is typical; fluorescence detection is not recommended. Blocking is with 3% normal serum with 0.1-0.2% triton X-100 usually. Primary antibody is diluted only in TBS or PBS with out blocking or triton X-100. Incubations are usually 3 hours 37C or 4C overnight. Detection is by ABC or PAP for best results. Not recommended for paraffin embedded tissues.
Optimal working dilutions must be determined by the end user.
Research CategoryEpigenetics & Nuclear Function
Research Sub CategoryChromatin Biology
Anti-Citrulline Antibody is a Rabbit Polyclonal Antibody for the detection of Citrulline & has been validated in ELISA & IHC.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Rabbit antiserum. Liquid with 0.05% sodium azide
Citrulline in glutaraldehyde fixed tissues or cells.
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio
(BSA) Bovine Serum Albumin
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.