Application
Immunohistochemistry: 1:1,000-1:2,500 glutaraldehyde required for reactivity. (0.5%-2.5% is typical; fluorescence detection is not recommended. Blocking is with 3% normal serum with 0.1-0.2% triton X-100 usually. Primary antibody is diluted only in TBS or PBS with out blocking or triton X-100. Incubations are usually 3 hours 37C or 4C overnight. Detection is by ABC or PAP for best results. Not recommended for paraffin embedded tissues.
ELISA: 1:2500-1:5000
Optimal working dilutions must be determined by the end user.
Research CategoryEpigenetics & Nuclear Function
Research Sub CategoryChromatin Biology
Anti-Citrulline Antibody is a Rabbit Polyclonal Antibody for the detection of Citrulline & has been validated in ELISA & IHC.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Citrulline-Glutaraldehyde-BSA
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form
Rabbit antiserum. Liquid with 0.05% sodium azide
Specificity
Citrulline in glutaraldehyde fixed tissues or cells.
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio
Citrulline-G-BSA 1
Arginine-G-BSA 1/>100,000
Glutamine-G-BSA 1/>100,000
Ornithine-G-BSA 1/>100,000
Abbreviations:
(G) Glutaraldehyde
(BSA) Bovine Serum Albumin
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Quantity
Est. Dispatch/Availability