Analysis Note

ControlHek293T co-expressing rat Bim_EL and active MEKK1 (MEKK1δ).

Application

Research CategoryApoptosis & Cancer

Anti-BimEL (pS69) human / (pS65) rat Antibody is an antibody against BimEL (pS69) human / (pS65) rat for use in WB.

Research Sub CategoryBCL2 & Inhibition

For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Bim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family. There are three isoforms of Bim: Bim_EL, Bim_L, and Bim_S. Bim is involved in regulating the intrinsic mitochondrial apoptotic pathway by inducing cytochrome c release, which in turn, activates caspase-9 and then caspase-3. Bim also plays a critical role in central and peripheral deletion of T lymphocytes and in controlling B cell homeostasis and activation. Bim_EL, the long isoform of Bim, is a ~28 kDa protein that is predominantly expressed in T and B cells and is activated by ERK1/2 pathway. The activation of Bim_EL by ERK1/2 promotes its phosphorylation on serine 65 (serine 69 in human), targeting it for degradation via the proteasome.

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of rat Bim_EL that contains serine 65 (serine 69 in the human sequence). The sequence is conserved in human and mouse.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide

Quality

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. The final product is generated by affinity chromatography using a Bim_EL-derived peptide that is phosphorylated at serine 65 (serine 69 in the human sequence).

Specificity

Rat Bim_EL when phosphorylated at serine 65. Human Bim_EL when phosphorylated at serine 69. Mouse Bim_EL (100% conserved) has not been tested, but is expected to react.

Storage and Stability

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.