Application

HABA/Avidin Reagent is suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples.

Reagent is capable of detecting biotin at µMolar concentrations (i.e., 1 nmole per ml). Typically used to quantitate biotin in biotinylated compounds.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

HABA/Avidin Reagent is specific for chromogenic applications that depend upon quantification of biotin.

4-Hydroxyazobenzene-2-carboxylic acid (HABA) is an avidin-binding dye that can be stoichiometrically displaced by biotin. The subsequent change in absorption at A500 provides a basis for spectrophotometric determination of biotin levels in biological samples.

Reconstitution

Prepares a ready-to-use HABA/avidin solution in buffered saline, pH approx. 7.3, with preservative when reconstituted with 10 ml deionized water.