Application

This Anti-unmethylated Histone H3 (Lys9) Antibody, clone 9B1-2G6 is validated for use in Immunocytochemistry for the detection of unmethylated Histone H3 (Lys9).

Research CategoryEpigenetics & Nuclear FunctionEpigenetics & Nuclear Function

See data sheet for Cat. No. MABE263, Anti-unmethylated Histone H3 (Lys9), clone 9B1-2G6, for additional Western blot and dot blot specificity analysis performed using an unconjugated version of this antibody.

Research Sub CategoryHistonesChromatin Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2. While lysine demethylation is mediated by enzymes such as JmjC domain-containing proteins.

Immunogen

KLH-conjugated linear peptide corresponding to human unmethylated Histone H3 (Lys9).

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified conjugated mouse monoclonal antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.

Protein G Purified

Quality

Immunocytochemistry Analysis:

A 1:50 dilution of this antibody detected unmethylated Histone H3 (Lys9) in HeLa cells.
Alexa Fluor^® is a registered trademark of Life Technologies™.

Specificity

This antibody recognizes Histone H3 unmethylated at Lys9. This antibody does not cross react with methyl-Histone (Lys29). This antibody detects unmethylated Histone H3 (Lys9) when Ser10 is phosphorylated.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~15 kDa observed. Molecular weight was observed in parent product MABE263