Application

Research Sub CategoryHistones

Immmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected phospho Histone H3 (Thr11) in HeLa cells.

Dot Blot Analysis: A 1:5,000 dilution from a representative lot detected phospho Histone H3 (Thr11) in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

Dylight™ is a registered trademark of Thermo Fisher Scientific.

This Anti-phospho Histone H3 (Thr11) Antibody, clone N123/48 is validated for use in western blotting, ICC & dot blot for the detection of phospho Histone H3 (Thr11).

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation.

Immunogen

Linear peptide corresponding to rat Histone H3 phosphorylated at Thr11.

Linkage

Replaces: MABE468

Physical form

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in Colcemid treated and untreated HeLa acid extract.

Western Blotting Anlaysis: 1:5,000 dilution of this antibody detected phospho Histone H3 (Thr11) in Colcemid treated HeLa acid extract and not in untreated HeLa acid extract.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~17 kDa observed