AntiGlucose6PhosphateIsomeraseClone1B7D7

Analysis Note

ControlHepG2, SMMC-7721, and rat liver lysates

Application

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GPI in human kidney tissue.

Immunofluorescent Analysis: A 1:200-1,000 dilution from a representative lot detected GPI in L-02 cells.

Optimal working dilutions must be determined by end user.

Anti-GPI Antibody, clone 1B7D7 is a highly specific mouse monoclonal antibody, that targets Glucose-6-phosphate isomerase & has been tested in western blotting, IHC & Immunofluorescence.

General description

Glucose-6-phosphate isomerase (GPI) alternatively known as Autocrine motility factor (AMF), Neuroleukin (NLK), Phosphoglucose isomerase (PGI), Phosphohexose isomerase (PHI) or Sperm antigen 36 (SA-36) and encoded by the gene name GPI is major enzyme in glycolysis and gluconeogenesis. Glucose-6-phosphate isomerase rearranges glucose-6-phosphate (from the phosphorylation of glucose via hexokinases) into fructose-6-phosphate (F6P) as the second step in glycolysis. Interestingly however, Glucose-6-phosphate isomerase plays an entirely different sort of role outside of the cell than when it is inside being part of glycolysis. When secreted, Glucose-6-phosphate isomerase acts as an autocrine motility factor for various cancers and is critical for metastasis, while when secreted in nervous tissue it acts as a neurotrophic factor for sensory and spinal neurons and in lymphocytes it acts as a lymphokine upon T cell ligand binding and stimulates B cells to secrete immunoglobulin. Mutations in Glucose-6-phosphate isomerase are the second most frequent cause of inherited glycolytic-enzymopathy in humans. This autosomal recessive disorder is characterized by anon-spherocytic anemia of variable severity which can present with neuromuscular dysfunctions defined by muscle weakness and mental retardation. EMD-Millipore's Anti-Glucose-6-phosphate isomerase monoclonal antibody has been tested in western blot on recombinant protein as well as cell lysates from HepG2, SMMC-7721 and rat liver tissue and in paraffin embedded immunohistochemistry on human kidney and fluorescent immunocytochemistry on L-O2 cells in culture.

Immunogen

Purified recombinant fragment of human GPI expressed in E. Coli.

Quality

Evaluated by Western Blotting in HepG2, SMMC-7721, and rat liver lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPI in HepG2, SMMC-7721, and rat liver lysates.

Target description

~56 kDa observed. Uncharacterized bands may appear in some lysate(s).