Application

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

This Anti-phospho-NUMA1 Antibody (Thr2055) is validated for use in Western Blotting and Peptide Inhibition Assay for the detection of phospho-NUMA1 (Thr2055).

Peptide Inhibition Analysis: A 1:500 dilution from a representative lot blocked NUMA1 phosphorylated at Thr2055 in Hydroxyurea treated HeLa nuclear extract.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NUMA1 or NUMA protein is an interesting and major component of the nuclear matrix of eukaryotic cells. NUMA1 is a highly abundant protein component that appears to serve a non-structural role but is needed for mitotic spindle poles and centrosome formation as well as a linker of microtubules. These functions are important for proper cytokinesis and the setup of the proper polarity axis during cell division. NUMA1 resides in the nuclear matrix during interphase. NUMA1 dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via a dynein/dynamin complex, and it remains there until the anaphase onset. Thus NUMA1 then can be found in the nucleus during cell rest and cytoplasmically and centrosome localized during cytokinesis. NUMA1 phosphorylation occurs by CDK1 and phosphorylation increases solubility and promotes association with dynein and subsequent translocation to the spindle poles.

Immunogen

Epitope: Tail Region

KLH-conjugated linear peptide corresponding to the tail region of human NUMA1 phosphorylated at Thr2055.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa nuclear extract and Hydroxyurea treated HeLa nuclear extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected NUMA1 phosphorylated at Thr2055 in 10 µg of HeLa nuclear extract and Hydroxyurea treated HeLa nuclear extract.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~230 kDa observed