Analysis Note

Positive ControlHeLa cells

Application

Immunoblotting (2.5-5 µg/ml)r>Immunocytochemistry (2.5-5 µg/ml)r>Paraffin Sections (not recommended)

General description

Recognizes both the ~55 kDa c-Fos and ~62 kDa v-Fos proteins. Does not cross-react with the ~39 kDa Jun protein.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with AG.8653X mouse myeloma cells. Recognizes the ~55 kDa c-fos and ~62 kDa v-fos proteins.

This Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of c-Fos (Ab-1).

Immunogen

a synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Recognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems.

Jensen, D.E., et al. 1991. Oncogene6, 1219.Reiss, M., et al. 1990. Cancer Res.50, 6641.RouscherIII, F.J., et al. 1988. Science240, 1010.Sassone-Corsi, P., et al. 1988. Cell54, 553.Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.Verma, I.M., et al. 1987. Cancer Res.49, 29.Verma, I.M., et al. 1987. Cell51, 513.Muller, R. 1986. Viochem. Biophys. Acta.823, 207.Verma, P. 1986. Trends Genet.2, 93.Greenberg, M., et al. 1984. Nature (London)311, 433.

Packaging

200 µg in Glass bottle

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Warning

Toxicity: Standard Handling (A)