Application

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Anti-acetyl Tubulin (Lys40) Antibody is an antibody against acetyl Tubulin (Lys40) for use in western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tubulin is the major constituent of microtubules, and microtubules are major cytoskeletal elements. Tubulin proteins undergo a number of post-translational modifications both during individual synthesis and after complete tubule formation. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects the affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

Immunogen

Epitope: Near N-Terminus

KLH-conjugated linear peptide corresponding to a region near the near N-Terminus of human Tubulin acetylated at Lys40.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in untreated and Trichostatin A treated HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected acetyl Tubulin (Lys40) in 10 µg of Trichostatin A treated HeLa cell lysate, and not in untreated HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~50 kDa observed