Analysis Note

ControlHeLa immunoprecipitation with rabbit IgG and Anti-WASH complex subunit 7

Application

Research CategoryCell Structure

Detect WASH complex subunit 7 (SWIP) using this Anti-WASH complex subunit 7 (SWIP) Antibody validated for use in IP.

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

WASH complex subunit 7 (SWIP) is a component of the WASH complex belonging to the WASH S7 family. The WASH complex is localized at early endosomal subdomains involved in the activation and recruitment the ARP 2/3 complex in order to stimulate actin polymerization and tubulin interaction. Through its interaction with FAM21, WASH plays a regulatory role in the retromer-mediated trafficking of vesicles. It is also involved in the tubule fission, components required in endosomal sorting processes as transport intermediates.

Immunogen

GST-tagged recombinant protein corresponding to human WASH complex subunit 7.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Immunoprecipitation/Western Blot Analysis in HeLa immunoprecipitation with rabbit IgG and Anti-WASH complex subunit 7.

Immunoprecipitation/Western Blot Analysis: 5 µg/mL of this antibody detected WASH complex subunit 7 in 10 µg of HeLa immunoprecipitation with rabbit IgG and Anti-WASH complex subunit 7.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~135 kDa observed.
IgG heavy chain may be observed at approximately 50 kDa