Analysis Note

ControlHuman placenta lysate

Application

This Anti-Transthyretin Antibody, clone 7G8-E11-E7 is validated for use in ELISA, WB for the detection of Transthyretin.

General description

Transthyretin is one of the three major thyroid hormone-binding proteins in plasma and/or cerebrospinal fluid of vertebrates. It exists mainly as a homotetrameric protein of approximately 55 kDa in plasma and, in sera and cerebrospinal fluid, is a carrier of the thyroid hormone thyroxine (T4) and retinol. While mostly known for being the transporter of thyroxine and retinol, when mutated, TTR is also well-described as the cause of familial amyloid polyneuropathy, a neurodegenerative lethal disorder characterized by systemic deposition of TTR amyloid fibrils, particularly in the peripheral nervous system. Recently, studies have shown that TTR has an important function development of peripheral and central nervous system physiology, namely by participating in behavior, in the maintenance of normal cognitive processes during ageing, amidated neuropeptide processing and nerve regeneration. Additionally, it has been proposed that TTR is neuroprotective in Alzheimer′s disease, by preventing the formation of amyloid beta fibrils (Fleming C.E. et al. (2009) Prog Neurobiol., Epub ahead of print

Immunogen

Purified human Transthyretin.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Evaluated by Western Blot in human placenta lysate.

Western Blot Analysis: 2 µg/ml of this antibody detected Transthyretin in 10 µg of human placenta lysate.

Specificity

This antibody recognizes Transthyretin.

Target description

Transthyretin exists mainly as a homotetrameric protein of approximately 55 kDa in plasma. A single band of ~26 kDa was observed, which is an approximate size of a dimeric component that makes up the said homotetramer.