Analysis Note

ControlHuman recombinant TIP60 protein

Application

Research Sub CategoryTranscription Factors

Research CategoryEpigenetics & Nuclear Function

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected TIP60 in liver carcinoma, rectum carcinoma, normal medulla and interbrain tissues.

Optimal working dilutions must be determined by end user.

Anti-TIP60 Antibody, clone 3F9 is a highly specific mouse monoclonal antibody, that targets Tip60 & has been tested in western blotting & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

TIP60 protein or also known as Histone acetyltransferase KAT5, 60kDa Tat-interactive protein, Histone acetyltransferase HTATIP, HIV-1 Tat interactive protein, Lysine acetyltransferase 5, or cPLA(2)-interacting protein and encoded by the KAT5/HTATIP/TIP60 gene is a histone acetyl transferase that forms the catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of a number of genes via the acetylation of histone H4 and H2A. TIP60 plays a role in DNA repair, growth arrest and senescence, apoptosis, chromatin remodeling, and tumor suppression. First discovered as protein that interacted with HIV-1 Tat resulting in TIP60 becoming polyubiquitinated and degraded (which prevented apoptosis of the infected cell), TIP60 is now known to interact with a number of other intracellular proteins including HDAC7A, CREB1, BCL3, Androgen receptor and PLA2G4A. TIP60 is largely localized to the nucleus or nuclear envelope in most tissues. TIP60 is also necessary for oncogene and proto-oncogene mediated growth induction seen in many cancers. EMD-Millipore's Anti-TIP60 mouse monoclonal antibody has been tested in western blot on recombinant TIP60 protein and in paraffin embedded immunohistochemistry on human liver carcinoma, rectum carcinoma, normal medulla and normal interbrain tissue sections.

Immunogen

Purified recombinant fragment of human TIP60 expressed in E. Coli.

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in human recombinant TIP60 protein.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected human recombinant TIP60 protein.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~60 kDa observed. Uncharacterized bands may appear in some lysate(s).