Application

Research CategoryNeuroscience

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Nkx6.1 in human pancreas and mouse pancreas tissue sections.

Anti-Nkx6.1, Cat. No. ABN1459, is a highly specific rabbit polyclonal antibody that targets Homeobox protein Nkx-6.1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Homeobox protein Nkx-6.1 (UniProt: P78426; also known as Homeobox protein NK-6 homolog A) is encoded by the NKX6-1 (also known as NKX6A) gene (gene ID: 4825) in human. Nkx-6.1 is a transcription factor that binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. It is expressed in pancreatic beta cells and is involved in transcriptional regulation in islet beta cells. During pancreatic development both Pdx-1 and Nkx-6.1 are observed in rat pancreas. However, later Nkx-6.1 became more beta-cell-restricted, and Pdx-1 is found in other islet cell types and in the duodenal epithelium. Nkx-6.1 binds to the insulin promoter and is involved in regulation of the insulin gene. The C-terminal domain of Nkx-6.1 contributes to sequence-specific DNA-binding. Together with NKX2-2 and IRX3 it acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by Sonic hedgehog (Shh) signals. Although Shh signaling is required for Nkx-6.1 expression in the ventral neural tube and spinal meninges, a Shh-independent pathway appears to operate in regulating Nkx-6.1 expression in the foregut region. (Ref.: Cai, J., et al. (2000). Genesis. 27(1); 6-11; Oster, A., et al. (1998). J. Histochem. Cytochem. 46(6); 707-715).

Immunogen

GST/His-tagged recombinant fragment corresponding to 61 amino acids from the N-terminal region of human Homeobox protein Nkx-6.1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in PANC-1 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Nkx6.1 in PANC-1 cell lysate.

Specificity

This rabbit polyclonal antibody detects Homeobox protein Nkx-6.1 in human cells. It targets an epitope within 61 amino acids from the N-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~54 kDa observed; 37.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).