Analysis Note

ControlHuman placenta tissue lysate

Application

Anti-Neutral amino acid transporter (ASCT2) Antibody detects level of Neutral amino acid transporter (ASCT2) & has been published & validated for use in WB, IH(P).

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Immunohistochemistry Analysis: 1:1000 dilution from a previous lot detected Neutral amino acid transporter in human prostate tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Neutral amino acid transporter, also known as ASCT2, is an alternate of the ASC (alanine-, serine-, cysteine-) transport system and is highly expressed in rat astroglia-rich cultures while not highly expressed in rat neuron-rich cultures. ASCT2 is also thought to be important in the glutamine release from cells. ASCT2 has been implicated in the growth and survival of cancer cells, therefore making it a possible target for cancer therapy. ASCT2 is thought to be a crucial neuronal neutral amino acid transporter.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of Neutral amino acid transporter.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Neutral amino acid transporter on 10 µg of human placenta tissue lysate.

Specificity

This antibody recognizes Neutral amino acid transporter at the N-terminus.

No homology found to mouse or rat. Other homologies: rhesus monkey (93% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 57 kDa observed