Anti-MGAT2, clone 4A5.2

Application

Research CategorySignaling

Detect MGAT2 using this mouse monoclonal Anti-MGAT2 Antibody, clone 4A5.2, Cat. No. MABS1703, validated for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC 2.4.1.143; UniProt Q10469; also known as Beta-1,2-N-acetylglucosaminyltransferase II, GlcNAc-T II, GNT-II, Mannoside acetylglucosaminyltransferase 2, MGAT2, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyl-transferase II) is encoded by the MGAT2 (also known as CDG2A) gene (Gene ID 4247) in human. MGAT2 is a Golgi enzyme that catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans. It possesses the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. It is highly expressed in small intestine in mice and humans. Mutations in MGTA2 gene are known to cause congenital disorders of glycosylation 2A, which is a multi-system disorder caused by defective glycoprotein biosynthesis. It leads to under-glycosylated levels of serum glycoproteins that result in defects in neuronal development, coagulation disorders, hypotonia, and immunodeficiency.

Ref.:
Tan, J., et al. (1995). Eur. J. Biochem. 231, 317-328.
Tan J., et al. (1996), Am. J. Hum. Genet. 159, 810-817.

Immunogen

GST-tagged internal fragment from the C-terminal half of human MGAT2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G purified.

Format: Purified

Quality

Evaluated by Western Blotting in T47D cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected MGAT2 in 10 µg of T47D cell lysate.

Specificity

Clone 4A5.2 targets an internal epitope within the C-terminal half of human MGAT2.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~52 kDa observed. 51.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).