Analysis Note
ControlHuman colon tissue lysate
Application
Anti-LEMD3 Antibody is a highly specific rabbit polyclonal antibody, that targets LEM domain-containing protein 3 & has been tested in western blotting & IHC.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected LEMD3 in human colon tissue.
Research CategorySignaling
Research Sub CategoryDevelopmental Signaling
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
LEM domain-containing protein 3 (LEMD3), also known as Inner nuclear membrane protein Man1 (MAN1), is an inner nuclear membrane protein that interacts with BMP and activin-TGF-beta receptor-activated Smads and antagonizes TGF-beta signaling in human cells. Additionally, LEMD3 is shown to Interact with SMAD1, SMAD2, SMAD3 and SMAD5. Studies also show that LEMD3 binds to both the phosphorylated and the unphosphorylated R-SMADS. LEMD3 is expressed in heart, brain, placenta, lung, liver and skeletal muscle. Defects in LEMD3 are shown to cause disorders, such as osteopoikilosis, Buschke-Ollendorff syndrome (BOS), and melorheostosis (MEL).
Immunogen
KLH-conjugated linear peptide corresponding to human LEMD3.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Quality
Evaluated by Western Blotting in human colon tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected LEMD3 in 15 µg of human colon tissue lysate.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Target description
~120 kDa observed. Uncharacterized bands may appear in some lysate(s).
This product has met the following criteria to qualify for the following awards: