Application

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected DDX41 in 10 µg of HeLa cell lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected DDX41 in human ovarian cancer, human cervical cancer, and human prostate cancer tissue.

Research CategoryInflammation & Immunology

This mouse monoclonal Anti-DDX41 antibody, clone 4D8.2, Cat. No. MABF1107 is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of DDX41.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Probable ATP-dependent RNA helicase DDX41 (UniProt: Q9UJV9; also known as DEAD box protein 41; DEAD box protein abstrakt homolog) is encoded by DDX41 (also known as ABS) gene (Gene ID 51428) in human. DDX41 belongs to the family of DEAD box helicase family of proteins, characterized by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif. DDX41 is reported to sense DNA virus in myeloid dendritic cells (mDCs) mediated by the adaptor STING (stimulator of interferon genes). DDX41 is reported to bind to both DNA and STING through a DEADc domain and then DDX41-STING complex localizes to the cytoplasm. DDX41 binds bacterial DNA in the infected cells and is shown to be more important than gamma-interferon-inducible protein IFI16 in the initial sensing of cytosolic B-form DNA in triggering the early burst of type I interferon response. Knockdown of DDX41 expression blocks the ability of mDCs to mount type I interferon and cytokine responses to DNA and DNA viruses. Ref.: Zhang, Z., et al. (2011). Nat. Immunol. 12(10): 959-966.

Immunogen

GST-tagged recombinant fragment corresponding to 115 amino acids from the N-terminal region of human DDX41.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HEPG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected DDX41 in 10 µg of HEPG2 cell lysate

Specificity

Clone 4D8.2 detects DDX41 protein in HEPG2 cells by Western blotting and in human ovarian and cervical cancer tissue by immunohisotchemistry.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~70 kDa observed; 69.8 kDa calculated. Uncharacterized bands may be observed in some lysate(s).