Analysis Note

ControlHEK293 cell lysate, PC12 cell lysate, NIH/3T3 cell lysate

Application

Research CategorySignaling

Western Blot Analysis: 0.5-2 µg/mL of this lot detected beta catenin in HEK293 cell lysate.

Research Sub CategoryDevelopmental Signaling

This Anti-Beta Catenin Antibody, clone 7F7.2 is validated for use in WB, IC for the detection of Beta Catenin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as α-catenin and β-catenin. α-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. When β-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in β-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When β-catenin is not associated with cadherins and α-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC). Recent evidence suggests that β-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy, HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.

Immunogen

Full length, GST-tagged recombinant beta catenin.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1002

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Routinely evaluated by western blot on HEK293 cell lysate.

Specificity

This antibody recognizes beta catenin.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

92 kDa