Analysis Note

Positive ControlEpithelial cells

Application

ELISA (0.5-1.0 µg/ml)r>Immunoblotting (1-10 µg/ml)r>Immunofluorescence (2-20 µg/ml)r>Immunohistochemistry (2-20 µg/ml)

General description

Recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts.

Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. It is validated for ELISA, Western blotting, immunofluorescence, and immunohistochemistry.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa AQP5 protein.

Immunogen

a synthetic peptide corresponding to a 17-amino acid sequence in the cytoplasmic region of rat AQP5

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lee, M.D., et al. 1996. J. Biol. Chem. 271, 8599.Raina, S., et al. 1995. J. Biol. Chem. 270, 1908.

Does not cross-react with other AQP proteins. The use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended in order to suppress non-specific bands. Variables associated with assay conditions will dictate the optimal working dilution.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)