Anti-ApoA4

Analysis Note

ControlHuman liver tissue lysate

Application

Anti-ApoA4 Antibody is a highly specific rabbit polyclonal antibody, that targets Apolipoprotein A & has been tested in western blotting & IHC.

Research CategorySignaling

Research Sub CategoryLipid Metabolism & Weight Regulation

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected ApoA4 in human colon tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ApoA4, also known as Apolipoprotein A4 or ApoA-IV, is a member of the apolipoprotein A1/A4/E family. ApoA4 is a major component of HDL and it can be found on the surface of newly synthesized chylomicrons. Studies have shown that APoA4 is required for efficient activation of lipoprotein lipase by ApoC-II, which is a potent activator of Lecithin Cholesterol Acyltransferase (LCAT). Other evidence points out that ApoA4 may have a role in chylomicrons and VLDL secretion and catabolism. The physiological function of ApoA4 is not confirmed yet, but evidence from several in vitro studies indicate that it may play a role in the reverse cholesterol transport pathway, transporting cholesterol from peripheral cells back to the liver. ApoA4 is synthesized primarily in the intestine and secreted in the plasma.

Immunogen

KLH-conjugated linear peptide corresponding to human ApoA4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected ApoA4 in 15 µg of human liver tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~38 kDa observed. Uncharacterized bands may appear in some lysate(s).