Application

Research CategoryApoptosis & Cancer

Anti-ALIX, Cat. No. ABC1435, is a rabbit polyclonal antibody that detects Programmed cell death 6-interacting protein and has been tested ted for use in Immunocytochemistry and Western Blotting.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected ALIX in 10 µg of HEK293 cell lysate.

Immunocytochemistry Analysis: A representative lot detected ALIX in Immunocytochemistry applications (Morita, E., et. al. (2007). EMBO J. 26(19):4215-27).

Western Blotting Analysis: A representative lot detected ALIX in Western Blotting applications (Morita, E., et. al. (2007). EMBO J. 26(19):4215-27; Sandrin, V., et. al. (2013). Retrovirology. 10:104; Mercenne, G., et. al. (2015). Elife. 4; Zhai, Q., et. al. (2011). J Virol. 85(1):632-7).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Programmed cell death 6-interacting protein (UniProt: Q8WUM4; also known as PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95) is encoded by the PDCD6IP (also known as AIP1, ALIX, KIAA1375) gene (gene ID: 10015) in human. ALIX, the mammalian orthologue of xenopus Xp95 and yeast BRO1, is an abundant adaptor protein involved in diverse cellular processes, including actin-based cytoskeleton assembly, integrin-mediated cell adhesion, and ESCRT- (endosomal sorting complex required for transport-) dependent membrane trafficking. ALIX is a Class E VPS protein involved in concentration and sorting of cargo proteins of the multi-vesicular body (MVB) for incorporation into intralumenal vesicles that are generated by invagination and scission from the limiting membrane of the endosome. Sorting of endocytosed membrane proteins by MVBs requires coordinated cargo recognition and assembly of membrane-sculpturing machinery that buds cargo-loaded membrane vesicles into the lumen of endosomes. MVB-mediated sorting is driven by five distinct ESCRT complexes (ESCRT-0, -I, -II and -III, and the Vps4 complex) and associated proteins. ALIX and charged MVB protein 4 (CHMP4), the mammalian orthologue of yeast Snf7, are binding partners involved in a variety of ESCRT-III-mediated membrane-remodeling processes in mammalian cells, including retroviral budding, cytokinetic abscission, biogenesis of exosomes, plasma membrane repair, and ubiquitin-independent MVB sorting of protease-activated receptor 1 (PAR1). In the absence of binding partners, an intramolecular interaction between the Patch 2 region in the Bro1 domain (aa 3-392) and the TSG101-docking site (aa 717-720) in the Pro-rich domain (aa 717-860) locks ALIX in a conformation inaccessible for interaction with CHMP4 and retroviral Gag proteins.

Immunogen

His-tagged recombinant fragment corresponding to the first 716 amino acids of human ALIX.

Epitope: unknown

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in Hela cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ALIX in 10 µg of HeLa cell lysate.

Specificity

This rabbit polyclonal antibody detects Programmed cell death 6-interacting protein in human and murine cells.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~90 kDa observed; 96.02 kda calculated. Uncharacterized bands may be observed in some lysate(s).